Going 3D in cell culture creates a far more natural environment. That means that 3D cultures provide data that more closely mirror what goes on in physiological conditions. Even gene expression works more naturally in 3D, as compared to 2D cultures. Consequently, 3D cell culture is gaining ground in culture-based science from basic research, such as developmental biology, to clinical studies, such as drug discovery and breast-cancer research. 3D Cell Culture and Analysis, a new Wiley Essential Knowledge Briefing, describes this transformation in culturing cells.
The information in this Essential Knowledge Briefing applies to anyone who uses cell-culture techniques or wants to keep up with this fast-moving field. Readers will explore the various forms of 3D culture, as well as how the cultures are made and used. Most important, 3D Cell Culture and Analysis explains why 3D culture surpasses 2D approaches in many, but not all, ways.
- Exploring a brief history of cell culture in 2D and 3D.
- Learning basic protocols for making 3D cultures.
- Troubleshooting cultures.
- Learning about 3D culture’s basic and clinical applications.
- Envisioning future directions of these techniques.