With the ability to build up detailed 3D images of biological features at depths of up to 1mm without causing damage, multiphoton microscopy has proved adept at studying both biological samples and living organisms. As such, it is now a critical technique in life science disciplines such as neurophysiology, cell biology, embryology, cancer and immunology.
This Essential Knowledge Briefing provides an introduction to the various forms of multiphoton microscopy and their use in life science. It explains the operation of a typical multiphoton instrument and details the specific practical requirements for different fields of research. It gives examples of how multiphoton microscopy is currently being used by working scientists and previews how it may develop in the future.